The oxidative burst at fertilization is dependent upon activation of the dual oxidase Udx1.

نویسندگان

  • Julian L Wong
  • Robbert Créton
  • Gary M Wessel
چکیده

The sea urchin egg is a quiescent cell...until fertilization, when the egg is activated. The classic respiratory burst at fertilization is the result of prodigious hydrogen peroxide production, but the mechanism for this synthesis is not known. Here we quantitate the kinetics of hydrogen peroxide synthesis at a single-cell level using an imaging photon detector, showing that 60 nM hydrogen peroxide accumulates within the perivitelline space of each zygote. We find that the NADPH oxidation activity is enriched at the cell surface and is sensitive to a pharmacological inhibitor of NADPH oxidase enzymes. Finally, we show that a sea urchin dual oxidase homolog, Udx1, is responsible for generating the hydrogen peroxide necessary for the physical block to polyspermy. Phylogenetic analysis of the enzymatic modules in Udx1 suggests a potentially conserved role for the dual oxidase family in hydrogen peroxide production and regulation during fertilization.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Reactive oxygen species and Udx1 during early sea urchin development.

Sea urchin fertilization is marked by a massive conversion of molecular oxygen to hydrogen peroxide by a sea urchin dual oxidase, Udx1. This enzyme is essential for completing the physical block to polyspermy. Yet, its expression is maintained during development, as indicated by the presence of both Udx1 mRNA and Udx1 protein enriched at the surface of all non-mesenchymal blastomeres. When hydr...

متن کامل

Ca21-dependent p47phox translocation in hydroperoxide modulation of the alveolar macrophage respiratory burst

Zhou, Huanfang, Roger F. Duncan, Timothy W. Robison, Lin Gao, and Henry Jay Forman. Ca21-dependent p47phox translocation in hydroperoxide modulation of the alveolar macrophage respiratory burst. Am. J. Physiol. 273 (Lung Cell. Mol. Physiol. 17): L1042–L1047, 1997.—Oxidative stress produces dual effects on the respiratory burst of rat alveolar macrophages. Preincubation with hydroperoxide concen...

متن کامل

Ca2+-dependent p47phox translocation in hydroperoxide modulation of the alveolar macrophage respiratory burst.

Oxidative stress produces dual effects on the respiratory burst of rat alveolar macrophages. Preincubation with hydroperoxide concentrations [H2O2or tert-butyl hydroperoxide ( t-BOOH); <50 μM] enhances stimulation of the respiratory burst, whereas higher concentrations inhibit stimulation. Both the enhancement and inhibition are markedly attenuated by buffering t-BOOH-induced changes in intrace...

متن کامل

I-17: Failed Fertilization: from Molecular,Diagnostic and Clinical Perspectives

Low and/or total failed fertilization following ICSI is a disappointing effect that some couples may face during their treatment which may have severe social and economic consequences for these infertile couples. This phenomenon has been mainly attributed to failed oocyte activation post ICSI. Researchers have regarded this failure due to the absence or deficiency of sperm associated to oocyte ...

متن کامل

Potential role of NADPH-oxidase in early steps of lead-induced oxidative burst in Vicia faba roots.

The mechanism of oxidative burst induced by lead in Vicia faba excised roots was investigated by luminol-dependent chemiluminescence. Results showed that lead triggered a rapid and dose-dependent increase in chemiluminescence production. In this study, specific inhibitors of putative reactive oxygen species (ROS) sources were used to determine the mechanism of lead-induced ROS generation. This ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Developmental cell

دوره 7 6  شماره 

صفحات  -

تاریخ انتشار 2004